Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It is concluded that the 32-kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase. © 1989.
Taketani, S., Kohno, H., Yoshinaga, T., & Tokunaga, R. (1989). The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase. FEBS Letters, 245(1–2), 173–176. https://doi.org/10.1016/0014-5793(89)80215-7