Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. SE50/110

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Abstract

In the biosynthesis of the C7-cyclitol moiety, valienol, of the α-glucosidase inhibitor acarbose in Actinoplanes sp. SE50/110 various cyclitol phosphates, such as 1-epi-valienol-7-phosphate, are postulated precursors. In the cell extracts of Actinoplanes SE50/110 we found a new kinase activity which specifically phosphorylates 1-epi-valienol; other C7-cyclitol analogs were only weakly or not phosphorylated. The purified product of the kinase reaction turned out to be 1-epi-valienol-7-phosphate in analyses by nuclear magnetic resonance spectroscopy. The enzyme seems not to be encoded by an acb gene and, therefore, plays a role in a salvage pathway rather than directly in the de novo biosynthesis of acarbose. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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Zhang, C. S., Podeschwa, M., Block, O., Altenbach, H. J., Piepersberg, W., & Wehmeier, U. F. (2003). Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. SE50/110. FEBS Letters, 540(1–3), 53–57. https://doi.org/10.1016/S0014-5793(03)00222-9

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