Identification and characterisation of Aedes aegypti aldehyde dehydrogenases involved in pyrethroid metabolism

10Citations
Citations of this article
29Readers
Mendeley users who have this article in their library.

Abstract

Background: Pyrethroid insecticides, especially permethrin and deltamethrin, have been used extensively worldwide for mosquito control. However, insecticide resistance can spread through a population very rapidly under strong selection pressure from insecticide use. The upregulation of aldehyde dehydrogenase (ALDH) has been reported upon pyrethroid treatment. In Aedes aegypti, the increase in ALDH activity against the hydrolytic product of pyrethroid has been observed in DDT/permethrin-resistant strains. The objective of this study was to identify the role of individual ALDHs involved in pyrethroid metabolism. Methodology/Principal Findings: Three ALDHs were identified; two of these, ALDH9948 and ALDH14080, were upregulated in terms of both mRNA and protein levels in a DDT/pyrethroid-resistant strain of Ae. aegypti. Recombinant ALDH9948 and ALDH14080 exhibited oxidase activities to catalyse the oxidation of a permethrin intermediate, phenoxybenzyl aldehyde (PBald), to phenoxybenzoic acid (PBacid). Conclusions/Significance: ALDHs have been identified in association with permethrin resistance in Ae. aegypti. Characterisation of recombinant ALDHs confirmed the role of this protein in pyrethroid metabolism. Understanding the biochemical and molecular mechanisms of pyrethroid resistance provides information for improving vector control strategies. © 2014 Lumjuan et al.

Cite

CITATION STYLE

APA

Lumjuan, N., Wicheer, J., Leelapat, P., Choochote, W., & Somboon, P. (2014). Identification and characterisation of Aedes aegypti aldehyde dehydrogenases involved in pyrethroid metabolism. PLoS ONE, 9(7). https://doi.org/10.1371/journal.pone.0102746

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free