Identification of human plasma metabolites exhibiting time-of-day variation using an untargeted liquid chromatographymass spectrometry metabolomic approach

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Abstract

Although daily rhythms regulate multiple aspects of human physiology, rhythmic control of the metabolome remains poorly understood. The primary objective of this proof-of-concept study was identification of metabolites in human plasma that exhibit significant 24-h variation. This was assessed via an untargeted metabolomic approach using liquid chromatography–mass spectrometry (LC-MS). Eight lean, healthy, and unmedicated men, mean age 53.6 (SD ± 6.0) yrs, maintained a fixed sleep/wake schedule and dietary regime for 1 wk at home prior to an adaptation night and followed by a 25-h experimental session in the laboratory where the light/dark cycle, sleep/wake, posture, and calorific intake were strictly controlled. Plasma samples from each individual at selected time points were prepared using liquid-phase extraction followed by reverse-phase LC coupled to quadrupole time-of-flight MS analysis in positive ionization mode. Time-of-day variation in the metabolites was screened for using orthogonal partial leas...

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Ang, J. E., Revell, V., Mann, A., Mäntele, S., Otway, D. T., Johnston, J. D., … Raynaud, F. (2012). Identification of human plasma metabolites exhibiting time-of-day variation using an untargeted liquid chromatographymass spectrometry metabolomic approach. Chronobiology International, 29(7), 868–881. https://doi.org/10.3109/07420528.2012.699122

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