Immunogenicity analysis of triterpene glycoside from holothuria atra to detecting fas and BCL-2 protein on the SP-C1 cell of tongue carcinoma

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Abstract

Objective: The objective of this study is to assess the role of triterpene glycoside of Holothuria atra to induce the Fas and Bcl-2-regulated apoptosis in Supri’s Clone 1 (Sp-C1) cell of tongue carcinoma. Methods: The triterpene glycoside of H. atra was isolated by high-performance liquid chromatography. The Sp-C1 cell of tongue carcinoma was cloned by Dulbecco’s Modified Eagle Medium and cytotoxicity assay by 3-4-5-dimethylthiazol-2yl 2,5-diphenyltetrazolium bromide assay. Expression Fas and Bcl-2 protein were analysed by immunocytochemistry also apoptosis detected by double staining ethidium bromide acridine. The datum of studied was analyzed by one-way analysis of variance (ANOVA), significance (p<0.05), and strength correlation (p<0.001) with R=1. Results: The H. atra has triterpene glycoside, and in the dose of 4 mg/ml, it has been cytotoxic activities on the Sp-C1 (p<0.05), mortality 80%; inhibitory concentration 50 (IC50)=0.6 and anti-logarithm = 4. In general, the concentration of 2.5 mg/ml of triterpene glycoside has triggered the expression of Fas protein (active, 71%; moderate, 10%; and no-active, 27%), whereas the Bcl-2 protein (active, 59%; moderate, 14%; and no-active 27%). Statistically, both expressions of protein were significant (p<0.05). Triterpene glycoside caused the apoptosis of Sp-C1 cell (strong, 87%, and moderate, 13%). Conclusion: The triterpene glycoside has the properties of cytotoxicity, and apoptosis in the SP-C1 cell also could be triggering the expression of Fas and Bcl-2 proteins.

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Arma, U., Satari, M. H., Ilyas, S., Handayani, D., Primasari, A., Kasuma, N., … Gani, B. A. (2018). Immunogenicity analysis of triterpene glycoside from holothuria atra to detecting fas and BCL-2 protein on the SP-C1 cell of tongue carcinoma. Asian Journal of Pharmaceutical and Clinical Research, 11(6), 248–252. https://doi.org/10.22159/ajpcr.2018.v11i6.25065

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