The compartment of mouse B cell progenitors can be resolved into five developmentally related fractions by multicolor flow cytometry. Using this system and employing mutant mice in which the membrane exon of the μ chain, the λ5 gene, or the JH locus was inactivated by gene targeting, we found that expression of the pre-B cell receptor complex is necessary for the transition from the large CD43+ to the small CD43- pre-B cell stage. We report the occurrence of immunoglobulin heavy and light chain gene rearrangement at the stage of large B cell precursors. We show that neither the pre-B cell receptor complex nor any gene rearrangement in the heavy chain locus is required for the induction of κ light chain gene rearrangement in early B cell progenitors. © 1993.
Ehlich, A., Schaal, S., Gu, H., Kitamura, D., Müller, W., & Rajewsky, K. (1993). Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development. Cell, 72(5), 695–704. https://doi.org/10.1016/0092-8674(93)90398-A