Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.
Pinto, L., Mateus-Pinheiro, A., Morais, M., Bessa, J. M., & Sousa, N. (2012). Immuno-golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons. PLoS ONE, 7(3). https://doi.org/10.1371/journal.pone.0033114