Immuno-golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons

8Citations
Citations of this article
77Readers
Mendeley users who have this article in their library.

Abstract

Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.

Cite

CITATION STYLE

APA

Pinto, L., Mateus-Pinheiro, A., Morais, M., Bessa, J. M., & Sousa, N. (2012). Immuno-golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons. PLoS ONE, 7(3). https://doi.org/10.1371/journal.pone.0033114

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free