Impact of aerosol dust on xMAP multiplex detection of different class pathogens

0Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

© 2017 Kleymenov, Gushchin, Gintsburg and Tkachuk. Environmental or city-scale bioaerosol surveillance can provide additional value for biodefense and public health. Efficient bioaerosol monitoring should rely on multiplex systems capable of detecting a wide range of biologically hazardous components potentially present in air (bacteria, viruses, toxins and allergens). xMAP technology from Luminex™ allows multiplex bead-based detection of antigens or nucleic acids, but its use for simultaneous detection of different classes of pathogens (bacteria, virus, toxin) is questionable. Another problem is the detection of pathogens in complex matrices, e.g., in the presence of dust. In the this research, we developed the model xMAP multiplex test-system aiRDeTeX 1.0, which enables detection of influenza A virus, Adenovirus type 6 Salmonella typhimurium, and cholera toxin B subunit representing RNA virus, DNA virus, gram-negative bacteria and toxin respectively as model organisms of biologically hazardous components potentially present in or spreadable through the air. We have extensively studied the effect of matrix solution (PBS, distilled water), environmental dust and ultrasound treatment for monoplex and multiplex detection efficiency of individual targets. All targets were efficiently detectable in PBS and in the presence of dust. Ultrasound does not improve the detection except for bacterial LPS.

Cite

CITATION STYLE

APA

Kleymenov, D. A., Gushchin, V. A., Gintsburg, A. L., & Tkachuk, A. P. (2017). Impact of aerosol dust on xMAP multiplex detection of different class pathogens. Frontiers in Microbiology, 8(NOV). https://doi.org/10.3389/fmicb.2017.02341

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free