We show that importin β3 is essential for the nuclear import of L7. The import is mediated via the multifaceted basic amino acid clusters present in the NH 2-region of L7, and is RanGTP-dependent. Using a (EGFP) 3 reporter system and a FRAP assay, the role the individual clusters play as a functional NLS has been characterized, and each cluster was found to exhibit a different rate of real time nuclear uptake. We assume that having such a multiple NLS may provide L7 with preferential nuclear uptake. © 2010 Federation of European Biochemical Societies.
Chou, C. W., Tai, L. R., Kirby, R., Lee, I. F., & Lin, A. (2010). Importin β3 mediates the nuclear import of human ribosomal protein L7 through its interaction with the multifaceted basic clusters of L7. FEBS Letters, 584(19), 4151–4156. https://doi.org/10.1016/j.febslet.2010.08.044