Improved isolation of undersampled bacteriophages: Finding of distant terminase genes

50Citations
Citations of this article
64Readers
Mendeley users who have this article in their library.

Abstract

Isolation and characterization of new environmental bacteriophages are performed for (1) analyzing microbial evolution and ecology and (2) delivering biological therapy. The sampling of environmental bacteriophages appears, however, to be limited by the procedure (usually liquid enrichment culture) used to propagate them. An alternative, less competitive procedure is developed here for the purpose of isolating new bacteriophages. This procedure involves extraction directly into and then propagation in a dilute agarose gel. Adaptations of this procedure are used to avoid repeated isolation of the same bacteriophage. Some newly isolated bacteriophages grow so poorly that they appear inaccessible to liquid enrichment culture. Four comparatively high titer bacteriophages were isolated and characterized by a genomic sequence survey. Some had genomes with extremely distant relationships to those of other bacteriophages, based on a tree built from the large terminase genes. These methods find novel genomes by rapidly isolating and screening diverse bacteriophages. © 2004 Elsevier Inc. All rights reserved.

Cite

CITATION STYLE

APA

Serwer, P., Hayes, S. J., Zaman, S., Lieman, K., Rolando, M., & Hardies, S. C. (2004). Improved isolation of undersampled bacteriophages: Finding of distant terminase genes. Virology, 329(2), 412–424. https://doi.org/10.1016/j.virol.2004.08.021

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free