Improved methodology for assaying brassinosteroids in plant tissues using magnetic hydrophilic material for both extraction and derivatization

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Abstract

Background: Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of the previously reported methods are tedious and time-consuming due to multiple pretreatment steps. Therefore, it is of great significance to develop a method to increase the throughput and detection sensitivity of BR anal. Results: We established a novel anal. method of BRs based on magnetic solid phase extn. (MSPE) combined with in situ derivatization (ISD). TiO2-coated magnetic hollow mesoporous silica spere(TiO2/MHMSS) was served as a double identity- a microextn. sorbent and "microreactor" for the capture and derivatization of BRs in sequence. BRs were first extd. onto TiO2/MHMSS through hydrophilic interaction. The BR-adsorbed TiO2/MHMSS was then employed as a "microreactor" for the derivatization of BRs with 4-(N,N-dimethyamino)phenylboronic acid (DMAPBA). The MSPE-ISD method was simple and fast, which could be accomplished within 10 min. Furthermore, the derivs. of BRs showed better MS response because they were incorporated with tertiary amino groups. Uniquely, endogenous BRs were detected in only 100 mg fresh wt. plant tissue. Conclusion: Our proposed MSPE-ISD method for the detn. of endogenous BRs is rapid and sensitive. It can be applied to the anal. of endogenous BRs in 100 mg fresh plant tissue (Brassica napus L. (B. napus L)). The proposed strategy for plant sample prepn. may be extended to develop anal. methods for detn. of a wide range of analytes with poor MS response in other complex sample matrixes. [on SciFinder(R)]

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Ding, J., Wu, J. H., Liu, J. F., Yuan, B. F., & Feng, Y. Q. (2014). Improved methodology for assaying brassinosteroids in plant tissues using magnetic hydrophilic material for both extraction and derivatization. Plant Methods, 10(1). https://doi.org/10.1186/1746-4811-10-39

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