Mitochondrial DNA derived from Apis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks a BglII restriction site found in other types of honeybee. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplified product with BglII yields contrasting patterns between Africanized and other honeybees.
Crozier, Y. C., Koulianos, S., & Crozier, R. H. (1991). An improved test for Africanized honeybee mitochondrial DNA. Experientia, 47(9), 968–969. https://doi.org/10.1007/BF01929894