Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR

0Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

Abstract

Background: Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low temperature and relatively good alkali stability. In this study, to better meet the requirements, the alkali stability of PCL was further improved via directed evolution with error-prone PCR. Results: The mutant PCL (N157F) with an improved alkali stability was selected based on a high-throughput activity assay. After incubating at pH 11.0 for 120 min, N157F retained 70% of its initial activity, which was 23% higher than that of wild type PCL. Combined with the three-dimensional structure analysis, N157F exhibited an improved alkali stability under the high pH condition due to the interactions of hydrophilicity and β-strand propensity. Conclusions: This work provided the theoretical foundation and preliminary data for improving alkali stability of PCL to meet the industrial requirements, which is also beneficial to improving alkali-tolerance ability of other industrial enzymes via molecular modification. How to cite: Huang L, Zheng D, Zhao Y, et al. Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR. Electron J Biotechnol 2019;39. https://doi.org/10.1016/j.ejbt.2019.04.002

Cite

CITATION STYLE

APA

Huang, L., Zheng, D., Zhao, Y., Ma, J., Li, Y., Xu, Z., … Liu, Y. (2019). Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR. Electronic Journal of Biotechnology, 39, 91–97. https://doi.org/10.1016/j.ejbt.2019.04.002

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free