The influence of statistical variations in cell distribution on the proliferation of Madin-Darby canine kidney (MDCK-II) cells was analyzed. MDCK-II cells were cultivated in parallel on 4 quartz crystal resonators in a bioreactor chip. Results of proliferation experiments with different initial grade of confluence were compared. Light microscope pictures of the sensor surface and impedance spectra were continuously acquired. CFD simulation results of the bioreactor chip were employed to verify experimental conditions. Results show that especially at low confluency dissimilar development of non-confluent MDCK-II cell layers and resulting changes in acoustic load depend significantly on initial cell distribution. © 2009.
Cama, G., Bolaeva, K., Jacobs, T., Hartmann, M., Hirsch, S., Naumann, M., & Hauptmann, P. (2009). Influence of statistical variations in cell distribution on the proliferation kinetics of Madin-Darby canine kidney cells on quartz crystal resonator. In Procedia Chemistry (Vol. 1, pp. 373–376). https://doi.org/10.1016/j.proche.2009.07.093