Inhibition of cAMP-Dependent PKA Activates β2 -Adrenergic receptor stimulation of cytosolic Phospholipase A2 via Raf-1/MEK/ERK and IP3 -Dependent Ca2+ Signaling in atrial myocytes

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Abstract

We previously reported in atrial myocytes that inhibition of cAMP-dependent protein kinase (PKA) by laminin (LMN)-integrin signaling activates β2-adrenergic receptor (β2-AR) stimulation of cytosolic phospholipase A2 (cPLA2 ). The present study sought to determine the signaling mechanisms by which inhibition of PKA activates β2-AR stimulation of cPLA2 . We therefore determined the effects of zinterol (0.1 μM; zint-β2-AR) to stimulate ICa,L in atrial myocytes in the absence (+PKA) and presence (-PKA) of the PKA inhibitor (1 μM) KT5720 and compared these results with atrial myocytes attached to laminin (+LMN). Inhibition of Raf-1 (10 μM GW5074), phospholipase C (PLC; 0.5 μM edelfosine), PKC (4 μM chelerythrine) or IP3 receptor (IP3R) signaling (2 μM 2-APB) significantly inhibited zint-β2-AR stimulation of ICa,L in-PKA but not +PKA myocytes. Western blots showed that zint-β2-AR stimulation increased ERK1/2 phosphorylation in-PKA compared to +PKA myocytes. Adenoviral (Adv) expression of dominant negative (dn) -PKCα, dn-Raf-1 or an IP3 affinity trap, each inhibited zint-β2-AR stimulation of ICa,L in + LMN myocytes compared to control +LMN myocytes infected with Adv-βgal. In +LMN myocytes, zint-β2 -AR stimulation of ICa,L was enhanced by adenoviral overexpression of wild-type cPLA2 and inhibited by double S505A/S515A2 mutant compared to control +LMN myocytes infected with Adv-βgal. In-PKA myocytes depletion of intracellular Ca2+ stores by 5 μM thapsigargin failed to inhibit zint-β2 -AR stimulation of ICa,L via cPLA2 . However, disruption of caveolae formation by 10 mM methyl-β-cyclodextrin inhibited zint-β2 -AR stimulation of ICa,L in-PKA myocytes significantly more than in +PKA myocytes. We conclude that inhibition of PKA removes inhibition of Raf-1 and thereby allows β2 -AR stimulation to act via PKCα/Raf-1/MEK/ERK1/2 and IP3 -mediated Ca2+ signaling to stimulate cPLA2 signaling within caveolae. These findings may be relevant to the remodeling of β-AR signaling in failing and/or aging heart, both of which exhibit decreases in adenylate cyclase activity.

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Pabbidi, M. R., Ji, X., Maxwell, J. T., Mignery, G. A., Samarel, A. M., & Lipsius, S. L. (2016). Inhibition of cAMP-Dependent PKA Activates β2 -Adrenergic receptor stimulation of cytosolic Phospholipase A2 via Raf-1/MEK/ERK and IP3 -Dependent Ca2+ Signaling in atrial myocytes. PLoS ONE, 11(12). https://doi.org/10.1371/journal.pone.0168505

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