Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.

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Abstract

This study was designed to isolate and identify a potent inhibitory compound against nitric oxide (NO) production from the stem bark of Ulmus pumila L. Ethyl acetate fraction of hot water extract registered a higher level of total phenolics (756.93mg GAE/g) and also showed strong DPPH (IC 50 at 5.6μg/mL) and ABTS (TEAC value 0.9703) radical scavenging activities than other fractions. Crude extract and its fractions significantly decreased nitrite accumulation in LPS-stimulated RAW 264.7 cells indicating that they potentially inhibited the NO production in a concentration dependent manner. Based on higher inhibitory activity, the ethyl acetate fraction was subjected to Sephadex LH-20 column chromatography and yielded seven fractions and all these fractions registered appreciable levels of inhibitory activity on NO production. The most effective fraction F1 was further purified and subjected to 1 H, 13 C-NMR and mass spectrometry analysis and the compound was identified as icariside E 4 . The results suggest that the U. pumila extract and the isolated compound icariside E 4 effectively inhibited the NO production and may be useful in preventing inflammatory diseases mediated by excessive production of NO.

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Joo, T., Sowndhararajan, K., Hong, S., Lee, J., Park, S. Y., Kim, S., & Jhoo, J. W. (2014). Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L. Saudi Journal of Biological Sciences, 21(5), 427–435. https://doi.org/10.1016/j.sjbs.2014.04.003

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