To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 trisphosphate (caged-InsP3) together with the Ca2+ indicator Fluo-3 ??? and monitor Ca2+ redistribution. Photo-released InsP3 olicits [Ca2+], changes exhibiting several kinetics phases and threshold ??? oscillations were induced after a single InsP3 pulse. Autoregenerative Ca2+ transients could also be induced by injections of Ca2+ itself, demonstrating unequivocally the presence of a Ca2+-induced Ca2+-release mechanism in these cells. © 1990.
Peres, A. (1990). InsP3- and Ca2+-induced Ca2+ release in single mouse oocytes. FEBS Letters, 275(1–2), 213–216. https://doi.org/10.1016/0014-5793(90)81474-3