Insulin stimulation of glucose uptake and the transmembrane potential of muscle cells in culture

6Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

The membrane potential of L6 muscle cells was measured with the fluorescent dye bis-oxonol. Hyperpolarizations of up to 15 mV were caused by gramicidin (in N-methyl-D-glucamine+ medium), or by monensin or ionomycin. Depolarization was achieved with gramicidin (in Na+ medium), or with K+. Insulin did not change the resting membrane potential of - 70 mV, yet it effectively stimulated 2-deoxy-D-glucose uptake. Conditions that hyperpolarize the cells did not alter the basal rate of hexose uptake. Moreover, insulin was still capable of stimulating hexose uptake in depolarized cells. It is concluded that modulation of the membrane potential is probably not a signalling event in insulin stimulaton of hexose uptake. © 1986.

Cite

CITATION STYLE

APA

Klip, A., Ramlal, T., & Walker, D. (1986). Insulin stimulation of glucose uptake and the transmembrane potential of muscle cells in culture. FEBS Letters, 205(1), 11–14. https://doi.org/10.1016/0014-5793(86)80855-9

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free