We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis. © 2008 Elsevier Inc. All rights reserved.
Eguchi, J., Yan, Q. W., Schones, D. E., Kamal, M., Hsu, C. H., Zhang, M. Q., … Rosen, E. D. (2008). Interferon Regulatory Factors Are Transcriptional Regulators of Adipogenesis. Cell Metabolism, 7(1), 86–94. https://doi.org/10.1016/j.cmet.2007.11.002