Intraepithelial lymphocytes subsets in different forms of celiac disease

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Abstract

Aim: The enumeration of intraepithelial lymphocytes subsets (total, γδ, and CD3– IELs) by flow cytometry (FCM), named as IEL lymphogram, constitutes a useful tool for celiac disease (CD) diagnosis. The aim of this study was to quantify IELs by FCM and their diagnostic value to differentiate active, silent and potential CD. Methods: Prospective study of 60 active and 20 silent CD patients, and 161 controls in which duodenal biopsy and IEL quantification by FCM was performed. Results: Active and silent CD patients had significant higher levels of both total and γδ IELs than absent CD patients (P < 0.0001 and P < 0.0001, P = 0.012 and P < 0.011; respectively). Active and silent CD patients had significant lower levels of CD3– IELs than absent CD patients (P < 0.047 and P < 0.009, respectively). Moreover, they were lower in silent than in active CD patients (P = 0.002). Changes of IELs subsets were more marked in children than adults active CD. The optimal IEL lymphogram cut off values for active CD diagnosis were: ≥10, ≥15 and ≤9 %, and with better performance characteristics for silent CD: ≥ 11, ≥10 and ≤5 %. Conclusion: The evaluation of IELs subsets by FCM is useful to confirm diagnosis of active and silent CD.

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Sánchez-Castañon, M., Castro, B. G., Toca, M., Santacruz, C., Arias-Loste, M., Iruzubieta, P., … López-Hoyos, M. (2016). Intraepithelial lymphocytes subsets in different forms of celiac disease. Autoimmunity Highlights, 7(1). https://doi.org/10.1007/s13317-016-0085-y

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