Ionizing radiation-induced adaptive response in fibroblasts under both monolayer and 3-dimensional conditions

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Abstract

© 2015 Zhao et al. To observe the adaptive response (AR) induced by ionizing radiation in human fibroblasts under monolayer and 3-dimensional (3-D) condition. Three kinds of fibroblasts were cultured under both monolayer and 3-D condition. Immunofluorescent staining was used to detect the γ-H2AX foci and the morphological texture. Trypan blue staining was used to detect the cell death. Western blot was used to detect the expressions of γ-H2AX, p53 and CDKN1A/p21 (p21). We found that DNA damage increased in a dose-dependent and time-dependent manner after high doses of radiation. When cells were pretreated with a priming low dose of radiation followed by high dose radiation, DNA damage was attenuated under both monolayer and 3-D condition, and the adaptive response (AR) was induced. Additionally, the morphology of cells under monolayer and 3-D conditions were different, and radiation also induced AR according to morphological texture analysis. Priming low dose radiation induced AR both under monolayer and 3-D condition. Interestingly, 3-D microenvironment made cells more sensitive to radiation. The expression of p53 and p21 was changed and indicated that they might participate in the regulation of AR.

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Zhao, Y., Zhong, R., Sun, L., Jia, J., Ma, S., & Liu, X. (2015). Ionizing radiation-induced adaptive response in fibroblasts under both monolayer and 3-dimensional conditions. PLoS ONE, 10(3). https://doi.org/10.1371/journal.pone.0121289

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