Isolation of total DNA from bacteria and yeast

  • Marco L
  • Roberta F
6Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

Many procedures in molecular biology require the isolation of high quality genomic DNA. This study investigated a new method to extract DNA from Gram-negative, Gram-positive bacteria, Mycobacteria and yeasts. Guanidine thioisocyanate present in DNAzol is capable of binding DNA to silica particle column. Subsequently the silica with adsorbed DNA is washed to remove impurities and the clean DNA eluted in appropriate buffer. Results indicated that the new extraction method is simple and reproducible. This isolation technique is faster and easier to perform than the other conventional extraction methods. Finally the recovered DNA is of high quality and suitable for downstream applications. <br />(African Journal of Biotechnology: 2003 2(8): 251-253)

Cite

CITATION STYLE

APA

Marco, L., & Roberta, F. (2003). Isolation of total DNA from bacteria and yeast. African Journal of Biotechnology, 2(8), 251–253. https://doi.org/10.5897/ajb2003.000-1052

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free