The native one-chain tissue plasminogen activator from the human melanoma cell line (Bowes) occurs in two variants as demonstrated by sodium dodecylsulphate-polyacrylamide gel electrophoresis of reduced and carboxymethylated samples. The two variants were isolated by affinity chromatography on arginine-Sepharose using a guanidinium-HCl gradient. in the order of elution, the variants were designated tissue plasminogen activator I and II. Variant I was found to be 3000 Mr larger than variant II and the difference was found to reside in the N-terminal half of the molecule. No substantial difference in carbohydrate content nor in enzymatic activity was demonstrated. © 1982.
Rånby, M., Bergsdorf, N., Pohl, G., & Wallén, P. (1982). Isolation of two variants of native one-chain tissue plasminogen activator. FEBS Letters, 146(2), 289–292. https://doi.org/10.1016/0014-5793(82)80936-8