Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing. © 1995.
Marshall, J., Molloy, R., Moss, G. W. J., Howe, J. R., & Hughes, T. E. (1995). The jellyfish green fluorescent protein: A new tool for studying ion channel expression and function. Neuron, 14(2), 211–215. https://doi.org/10.1016/0896-6273(95)90279-1