Label-free detection of biomolecular interactions in real time with a nano-porous silicon-based detection method

19Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

Abstract

Background: We describe a biosensor platform for monitoring molecular interactions that is based on the combination of a defined nano-porous silicon surface, coupled to light interferometry. This platform allows the label-free detection of protein-protein and protein-DNA interactions in defined, as well as complex protein mixtures. The silicon surface can be functionalized to be compatible with traditional carboxyl immobilization chemistries, as well as with aldehyde-hydrazine bioconjugation chemistries. Results: We demonstrate the utility of the new platform in measuring protein-protein interactions of purified products in buffer, in complex mixtures, and in the presence of different organic solvent spikes, such as DMSO and DMF, as these are commonly used in screening chemical compound libraries. Conclusion: Nano-porous silicon, when combined with white light interferometry, is a powerful technique for the measurement of protein-protein interactions. In addition to studying the binary interactions of biomolecules in clean buffer systems, the newly developed surfaces are also suited for studying interactions in complex samples, such as plasma. © 2008 Latterich and Corbeil; licensee BioMed Central Ltd.

Cite

CITATION STYLE

APA

Latterich, M., & Corbeil, J. (2008). Label-free detection of biomolecular interactions in real time with a nano-porous silicon-based detection method. Proteome Science, 6. https://doi.org/10.1186/1477-5956-6-31

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free