Lactococcus lactis as host for overproduction of functional membrane proteins

146Citations
Citations of this article
176Readers
Mendeley users who have this article in their library.

Abstract

Lactococcus lactis has many properties that are ideal for enhanced expression of membrane proteins. The organism is easy and inexpensive to culture, has a single membrane and relatively mild proteolytic activity. Methods for genetic manipulation are fully established and a tightly controlled promoter system is available, with which the level of expression can be varied with the inducer concentration. Here we describe our experiences with lactococcal expression of the mechanosensitive channel, the human KDEL receptor and transporters belonging to the ABC transporter family, the major facilitator superfamily, the mitochondrial carrier family and the peptide transporter family. Previously published expression studies only deal with the overexpression of prokaryotic membrane proteins, but in this paper, experimental data are presented for the overproduction of mitochondrial and hydrogenosomal carriers and the human KDEL receptor. These eukaryotic membrane proteins were expressed in a functional form and at levels amenable to structural work. © 2002 Elsevier Science B.V. All rights reserved.

Cite

CITATION STYLE

APA

Kunji, E. R. S., Slotboom, D. J., & Poolman, B. (2003, February 17). Lactococcus lactis as host for overproduction of functional membrane proteins. Biochimica et Biophysica Acta - Biomembranes. Elsevier. https://doi.org/10.1016/S0005-2736(02)00712-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free