Chemical components act in plant defense and protection, but many of them are extracted and usedmedicinally. For Cerrado, active chemical components are used in the treatment of diseases, whichstrengthens the necessity for pharmacological studies of plants of that environment. The objective wasto evaluate the histochemistry of the leaf blade of Byrsonima verbascifolia (L.) DC., Malpighiaceae, Cam-pomanesia adamantium (Cambess.) O.Berg, Myrtaceae, Roupala montana Aubl., Proteaceae, and Solanumlycocarpum A. St.-Hil., Solanaceae, species that have been reported as producers of secondary metabolitesfor pharmacological use. The 3rdnode leaves (median, intercostal and margin regions) were collected, fixed, included in Paraplast®or 2-hydroxyethyl methacrylate, sectioned in microtome, stained and photo-graphed on microscope. This analysis aimed to find leaf regions which produced chemical compounds. For histochemical tests, intercostal areas were selected from median region leaf of the 3rdnode. Samplesfresh and newly collected and fixed and embedded in Paraplast®were used. Tests were conducted forlipids, terpenoids, phenolic compounds, alkaloids, sugars and proteins. Alkaloids were observed only inR. montana, as well as the results for phenolic compounds. Flavonoids are present in B. verbascifolia and R.montana. The lipid composition was showed for the chemical compounds of B. verbascifolia and C. adaman-tium, which proved to be part of the essential oils or resins oils in C. adamantium idioblasts. The chemicalcompounds of B. verbascifolia, C. adamantium and R. montana are present mainly in idioblasts among theparenchyma and epidermal cells. C. adamantium has secretory cavities, but only with lipid content. Theidentification of chemical compounds has not been possible in mature leaves of S. lycocarpum.
Kuster, V. C., & Vale, F. H. A. (2016). Leaf histochemistry analysis of four medicinal species from Cerrado. Brazilian Journal of Pharmacognosy, 26(6), 673–678. https://doi.org/10.1016/j.bjp.2016.05.015