Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor

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Abstract

Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of <sc>d</sc>-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing <sc>d</sc>-rhamnose and not <sc>d</sc>-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of <sc>d</sc>-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins. © 2014 McCaughey et al.

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McCaughey, L. C., Grinter, R., Josts, I., Roszak, A. W., Waløen, K. I., Cogdell, R. J., … Walker, D. (2014). Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor. PLoS Pathogens, 10(2). https://doi.org/10.1371/journal.ppat.1003898

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