Lef/Tcf-dependent Wnt/β-catenin signaling during Xenopus axis specification

Citations of this article
Mendeley users who have this article in their library.


Though the Wnt/β-catenin signaling pathway is known to play key roles during Xenopus axis specification, whether it signals exclusively through Lef/Tcf transcription factors in this process remains unclear. To investigate this issue, we generated transgenic frog embryos expressing green fluorescent protein (GFP) driven by a Lef/Tcf-dependent and Wnt/β-catenin-responsive promoter. This promoter is highly sensitive and even detects maternal β-catenin activity prior to the large-scale transcription of zygotic genes. Unexpectedly, GFP expression was observed only in some, but not all, known Wnt/β-catenin-positive territories in Xenopus early development. Furthermore, ubiquitous expression of dominant Lef-1 protein variants from transgenes revealed that zygotic Lef/Tcf activity is required for the ventroposterior development of Xenopus embryos. In summary, our results suggest that endogenous Wnt/β-catenin activity does not result in obligatory Lef/Tcf-dependent gene activation, and that the ventroposteriorizing activity of zygotic Wnt-8 signaling is mediated by Lef/Tcf proteins. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.




Geng, X., Xiao, L., Lin, G. F., Hu, R., Wang, J. H., Rupp, R. A. W., & Ding, X. (2003). Lef/Tcf-dependent Wnt/β-catenin signaling during Xenopus axis specification. FEBS Letters, 547(1–3), 1–6. https://doi.org/10.1016/S0014-5793(03)00639-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free