A linear surface epitope in a proline-rich region of ORF3 product of genotype 1 hepatitis E virus

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Abstract

© 2016 by the authors; licensee MDPI, Basel, Switzerland. Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.

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Yang, Y., Lin, S., Nan, Y., Ma, Z., Yang, L., & Zhang, Y. (2016). A linear surface epitope in a proline-rich region of ORF3 product of genotype 1 hepatitis E virus. Viruses, 8(8). https://doi.org/10.3390/v8080227

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