A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan ( Dipsacus asperoides ) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS. A C18 column was used with a 0.1% aqueous formic acid : methanol gradient mobile phase. The analytes were quantified 325 nm. With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced. The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63 mg/g, respectively, and the method LOD was 0.13 and 0.02 mg/g, respectively.
Pearson, J. L., Lee, S., Suresh, H., Low, M., Nang, M., Singh, S., … Khoo, C. S. (2014). The Liquid Chromatographic Determination of Chlorogenic and Caffeic Acids in Xu Duan ( Dipsacus asperoides ) Raw Herb . ISRN Analytical Chemistry, 2014, 1–6. https://doi.org/10.1155/2014/968314