Localization and Function of Budding Yeast CENP-A Depends upon Kinetochore Protein Interactions and Is Independent of Canonical Centromere Sequence

7Citations
Citations of this article
37Readers
Mendeley users who have this article in their library.

Abstract

In many eukaryotes, the centromere is epigenetically specified and not strictly defined by sequence. In contrast, budding yeast has a specific 125bp sequence required for kinetochore function. Despite the difference in centromere specification, budding yeast and multicellular eukaryotic centromeres contain a highly conserved histone H3 variant, CENP-A. The localization of budding yeast CENP-A, Cse4, requires the centromere DNA binding components, which are not conserved in multicellular eukaryotes. Here, we report that Cse4 localizes and functions at a synthetic kinetochore assembly site that lacks centromere sequence. The outer kinetochore Dam1-DASH and inner kinetochore CBF3 complexes are required for Cse4 localization to that site. Furthermore, the natural kinetochore also requires the outer kinetochore proteins for full Cse4 localization. Our results suggest that Cse4 localization at a functional kinetochore does not require the recognition of a specific DNA sequence by the CBF3 complex; rather, its localization depends on stable interactions among kinetochore proteins.

Cite

CITATION STYLE

APA

Ho, K. H., Tsuchiya, D., Oliger, A. C., & Lacefield, S. (2014). Localization and Function of Budding Yeast CENP-A Depends upon Kinetochore Protein Interactions and Is Independent of Canonical Centromere Sequence. Cell Reports, 9(6), 2027–2033. https://doi.org/10.1016/j.celrep.2014.11.037

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free