Lymphoblast-derived integration-free iPS cell line from a 65-year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant

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Abstract

Human lymphoblast cells from a male patient diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem (iPS) cells employing episomal plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. The iPS cells retained the TREM2 mutation, and were defined as pluripotent based on (i) expression of pluripotent-associated markers, (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptomes of the iPS cell line and the human embryonic stem cell line H1 with a Pearson correlation of 0.966.

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Schröter, F., Sleegers, K., Cuyvers, E., Bohndorf, M., Wruck, W., Van Broeckhoven, C., & Adjaye, J. (2016). Lymphoblast-derived integration-free iPS cell line from a 65-year-old Alzheimer’s disease patient expressing the TREM2 p.R47H variant. Stem Cell Research, 16(1), 113–115. https://doi.org/10.1016/j.scr.2015.12.017

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