Laboratory cultures of marine Synechococcus sp. WH 7803 were grown under conditions of restricted iron availability. The culture medium was adjusted to restrict iron availability: (i) by adding the iron chelator EDDA; (ii) by omitting iron; and (iii) by omitting both iron and EDTA, An adaptive response was observed to these iron-restricted conditions, including decrease in cellular phycoerythrin concentration and synthesis of a 36 kDa polypeptide in [35S]methionine radiolabelled whole cell lysates separated by SDS-PAGE. The polypeptide was synthesized within 48 h of transferring exponential phase cells to the iron-restricted medium. The protein was localized to the cell membranes and not the cytoplasmic fraction.
Chadd, H. E., Joint, I. R., Mann, N. H., & Carr, N. G. (1996). The marine picoplankter Synechococcus sp. WH 7803 exhibits an adaptive response to restricted iron availability. FEMS Microbiology Ecology, 21(2), 69–76. https://doi.org/10.1016/0168-6496(96)00031-1