Marking embryonic stem cells with a 2A self-cleaving peptide: A NKX2-5 emerald GFP BAC reporter

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Abstract

Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter constructs that preserve the regulatory sequences near a gene's native ATG start site has not been widely available.

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Hsiao, E. C., Yoshinaga, Y., Nguyen, T. D., Musone, S. L., Kim, J. E., Swinton, P., … Conklin, B. R. (2008). Marking embryonic stem cells with a 2A self-cleaving peptide: A NKX2-5 emerald GFP BAC reporter. PLoS ONE, 3(7). https://doi.org/10.1371/journal.pone.0002532

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