MESC and hiPSC proliferation on negative photoresists for microfluidics

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We report the effects on pluripotent stem cells proliferation of an antimony salt in chemically amplified negative photoresists (e.g., SU-8, TMMR) commonly used in MEMS fabrications. Since antimony and its derivatives are suspected to be toxic for mammalian cells including mESCs (mouse embryonic stem cells) and hiPSCs (human induced pluripotent stem cells), but it has not yet been investigated. Irrespective of antimony in photoresists, mESCs and hiPSCs cultured on photoresists coated with Matrigel were able to form their colonies and maintain at an undifferentiated state for 7-day culture. The successful 7-day culture is suggested to be ability for investigations of stem cells culture in microfluidic systems. © 2011 Published by Elsevier Ltd.




Hirai, Y., Kamei, K. I., Makino, Y., Liu, L., Yuan, Q., Chen, Y., & Tabata, O. (2011). MESC and hiPSC proliferation on negative photoresists for microfluidics. In Procedia Engineering (Vol. 25, pp. 1233–1236).

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