Methods for Generating Year-Round Access to Amphioxus in the Laboratory

10Citations
Citations of this article
32Readers
Mendeley users who have this article in their library.

Abstract

Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphioxus colonies in the laboratory and obtaining embryos at any time of day and over almost the entire year, far exceeding natural conditions. This is achieved by mimicking the natural benthic environment, natural day- and moon- light, natural substrate and by providing a strictly controlled and seasonally fluctuating temperature regimen. Moreover, supplemented algae diets allow animals to refill their gonads in consecutive years. Spontaneous spawning, a major problem in previous setups, no longer occurs in our facility; instead, all breeding is induced and fertilization occurs fully in vitro. Our system makes amphioxus a standard laboratory animal model. © 2013 Benito-Gutiérrez et al.

Cite

CITATION STYLE

APA

Benito-Gutiérrez, È., Weber, H., Bryant, D. V., & Arendt, D. (2013). Methods for Generating Year-Round Access to Amphioxus in the Laboratory. PLoS ONE, 8(8). https://doi.org/10.1371/journal.pone.0071599

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free