Molecular determinants for subcellular localization of PSD-95 with an interacting K+ channel

102Citations
Citations of this article
64Readers
Mendeley users who have this article in their library.

Abstract

Ion channels and PSD-95 are colocalized in specific neuronal subcellular locations by an unknown mechanism. To investigate mechanisms of localization, we used biolistic techniques to express GFP-tagged PSD95 (PSD-95:GFP) and the K+-selective channel Kv1.4 in slices of rat cortex. In pyramidal cells, PSD- 95:GFP required a single PDZ domain and a region including the SH3 domain for localization to postsynaptic sites. When transfected alone, PSD-95:GFP was present in dendrites but absent from axons. When cotransfected with Kv1.4, PSD-95:GFP appeared in both axons and dendrites, while Kv1.4 was restricted to axons. When domains that mediate the interaction of Kv1.4 and PSD-95 were disrupted, Kv1.4 localized nonspecifically. Our results provide evidence that Kv1.4 itself may determine its subcellular location, while an associated MAGUK protein is a necessary but not sufficient co factor.

Cite

CITATION STYLE

APA

Arnold, D. B., & Clapham, D. E. (1999). Molecular determinants for subcellular localization of PSD-95 with an interacting K+ channel. Neuron, 23(1), 149–157. https://doi.org/10.1016/S0896-6273(00)80761-8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free