Monitoring the identity of bacteria using their intrinsic fluorescence

Citations of this article
Mendeley users who have this article in their library.


Three different fluorescence spectra were recorded following excitation at 250 nm (aromatic amino acids+nucleic acids), 270 nm (tryptophan residues) and 316 nm (NADH) for 25 strains of bacteria in dilute suspensions. Evaluation of the spectra using principal component analysis and hierarchical clustering showed a good reproducibility from culture to culture and a good discrimination of the bacteria. Applying the method of Mahalanobis distances to the spectra of lactobacilli species recorded following excitation at 250 nm, a good classification was observed for 100% and 81% of calibration and validation groups, respectively. The developed method allows the discrimination and identification of the investigated bacteria at the genus, species and strain levels. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.




Leblanc, L., & Dufour, É. (2002). Monitoring the identity of bacteria using their intrinsic fluorescence. FEMS Microbiology Letters, 211(2), 147–153.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free