Monitoring the structural behavior of troponin and myoplasmic free Ca 2+ concentration during twitch of frog skeletal muscle

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Abstract

The interaction of troponin molecules on the thin filament with Ca 2+ plays a key role in regulating muscle contraction. To characterize the structural changes of troponin caused by Ca2+ and crossbridge formation, we recorded the small-angle x-ray Intensity and the myoplasmic free Ca2+ concentration using fluo-3 AM in the same frog skeletal muscle during twitch elicited by a single electrical pulse at 16°C. In an overstretched muscle, the intensity of the meridional reflection from troponin at 1/38.5 nm-1 began to change at 4 ms after the stimulus, reached a peak at 10 ms, and returned to the resting level with a halftime of 25 ms. The concentration of troponin-bound Ca2+ began to increase at 1-2 ms after the stimulus, reached a peak at 5 ms, and returned to the resting level with a halftime of 40 ms, indicating that troponin begins to change conformation only after a sizable amount of Ca2+ has bound to it, and returns to the resting structure even when there is still some bound Ca2+. In a muscle with a filament overlap, crossbridge formation appears to slow down Ca2+ release from troponin and have a large effect on its conformation. © 2010 by the Biophysical Society.

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Matsuo, T., Iwamoto, H., & Yagi, N. (2010). Monitoring the structural behavior of troponin and myoplasmic free Ca 2+ concentration during twitch of frog skeletal muscle. Biophysical Journal, 99(1), 193–200. https://doi.org/10.1016/j.bpj.2010.04.021

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