Genotyping methods developed to survey the transmission dynamics of Mycobacterium tuberculosis currently rely on the interpretation of restriction and amplification profiles. Multispacer sequence typing (MST) genotyping is based on the sequencing of several intergenic regions selected after complete genome sequence analysis. It has been applied to various pathogens, but not to M. tuberculosis.
Djelouadji, Z., Arnold, C., Gharbia, S., Raoult, D., & Drancourt, M. (2008). Multispacer sequence typing for Mycobacterium tuberculosis genotyping. PLoS ONE, 3(6). https://doi.org/10.1371/journal.pone.0002433