A new near-infrared fluorescent probe (NIR-PbP) for sensitive detection of Pb(II) ions in solution and living cells has been rationally designed and synthesized. The NIR-PbP is inherently non-fluorescent and gains fluorescence in the presence Pb(II) ions. The ion detection is based on Pb(II)-induced unmasking the fluorophore through the opening of the spyrocycle, with more than 500-fold fluorescence enhancement for sub-micromolar Pb(II) concentration. The NIR-PbP has high sensitivity, good photo-stability, low detection limit, and reversible response to Pb(II) ions.
Bi, J., Fang, M., Wang, J., Xia, S., Zhang, Y., Zhang, J., … Liu, H. (2017). Near-infrared fluorescent probe for sensitive detection of Pb(II) ions in living cells. Inorganica Chimica Acta, 468, 140–145. https://doi.org/10.1016/j.ica.2017.06.044