Nectandra falcifolia: Potential phytopharmaceutical for skin damage protection designed by statistical approach and characterized by photoacoustic spectroscopy

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Abstract

Phytopharmaceutical products are being used in the treatment and prevention of health problems.Nowadays, the development and evaluation of novel pharmaceutical products is expensive and time con-suming. A statistical approach is a good tool for optimal development processes. Nectandra falcifolia (Nees)J.A. Castigl. ex Mart. Crov. & Piccinini, Lauraceae, a Brazilian species, is reported as anti-inflammatory,anti-leishmanial and anti-microbial. However, there is little known about its chemical composition. Forother species of Nectandra genus, the presence of antioxidant compounds is reported. In order to opti-mize the process of obtaining extract with high antioxidant activity, different extraction conditions weretested following a statistical approach. Two sequential experimental designs were used – first, a factorial23design, followed by central composite 22. The extracts manufactured by these experimental statisticalmatrixes had their antioxidant activity and phenolic contents quantified and the response surface plotswere fitted in quadratic models and they predicted the best extraction condition for the best antioxidantactivity. This standardized extract and its antioxidant activity were better evaluated by two comple-mentary tests (ABTS and Burst respiratory). A topical formulation containing 1% (w/w) of standardizedextract was prepared and used for an in vivo skin permeation study using a two-dose application. Thephotoacoustic spectroscopy was used to analyze the samples from the permeation study and the com-position profile of standardized extract. In rat skin samples, the data demonstrated that for the higherdose of topical formulation (5 g/cm2), the standardized extract could cross skin and be seen in epidermisand dermis. This was not the case for the lower dose (2 g/cm2) which was only present in the epidermis.This information suggests that this novel standardized extract of N. falcifolia could be explored for skindamage prevention or treatment for diseases developed by oxidative damage.

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Borghi-Pangoni, F. B., Tunin, L. M., Bonifácio, K. L., Nogueira, A. C., Hernandes, L., Yamashita, F., … Diniza, A. (2015). Nectandra falcifolia: Potential phytopharmaceutical for skin damage protection designed by statistical approach and characterized by photoacoustic spectroscopy. Brazilian Journal of Pharmacognosy, 25(3), 284–291. https://doi.org/10.1016/j.bjp.2015.02.012

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