Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/ VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocyto-genes.
Bear, J. E., Loureiro, J. J., Libova, I., Fässler, R., Wehland, J., & Gertler, F. B. (2000). Negative regulation of fibroblast motility by Ena/VASP proteins. Cell, 101(7), 717–728. https://doi.org/10.1016/S0092-8674(00)80884-3