Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP

55Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

Cite

CITATION STYLE

APA

Polte, T., Oberle, S., & Schröder, H. (1997). Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP. FEBS Letters, 409(1), 46–48. https://doi.org/10.1016/S0014-5793(97)00480-8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free