Chlorophyll-a (Chl-a) was readily converted into Chl-d under mild conditions without any enzymes. Treatment of Chl-a dissolved in dry tetrahydrofuran (THF) with thiophenol and acetic acid at room temperature successfully produced Chl-d in 31% yield. During the acidic oxidation, removal of the central magnesium, pheophytinization, was sufficiently suppressed. This mild pathway can give insights into the yet unidentified Chl-d biosynthesis. Crown Copyright © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.
Fukusumi, T., Matsuda, K., Mizoguchi, T., Miyatake, T., Ito, S., Ikeda, T., … Oba, T. (2012). Non-enzymatic conversion of chlorophyll-a into chlorophyll-d in vitro: A model oxidation pathway for chlorophyll-d biosynthesis. FEBS Letters, 586(16), 2338–2341. https://doi.org/10.1016/j.febslet.2012.05.036