A novel cellular model to study angiotensin II at2 receptor function in breast cancer cells

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Abstract

Recent studies have highlighted the AT1 receptor as a potential therapeutic target in breast cancer, while the role of the AT2 subtype in this disease has remained largely neglected. The present study describes the generation and characterization of a new cellular model of human invasive breast cancer cells (D3H2LN-AT2) stably expressing high levels of Flag-tagged human AT2 receptor (Flag-hAT2). These cells exhibit high-affinity binding sites for AngII, and total binding can be displaced by the AT2-selective antagonist PD123319 but not by the AT1-selective antagonist losartan. Of interest, high levels of expression of luciferase and green fluorescent protein make these cells suitable for bioluminescence and fluorescence studies in vitro and in vivo . We provide here a novel tool to investigate the AT2 receptor functions in breast cancer cells, independently of AT1 receptor activation.

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Rodrigues-Ferreira, S., Morel, M., Reis, R. I., Cormier, F., Baud, V., Costa-Neto, C. M., & Nahmias, C. (2012). A novel cellular model to study angiotensin II at2 receptor function in breast cancer cells. International Journal of Peptides, 2012. https://doi.org/10.1155/2012/745027

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