A novel role of dickkopf-related protein 3 in macropinocytosis in human bladder cancer T24 cells

3Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

� 2016 by the authors; licensee MDPI, Basel, Switzerland. Dickkopf-related protein 3 (Dkk-3) is a potential tumor suppressor reported in various cancer entities. However, we found that Dkk-3 was exceptionally upregulated in bladder cancer T24 cells. To validate the biological role of Dkk-3 other than a tumor suppressor, we examined the function of Dkk-3 in T24 cells. Gene silencing of Dkk-3 inhibited cell growth through inducing G0/G1 cell-cycle arrest. Furthermore, Dkk-3 knock-down caused macropinocytosis accompanied by autophagy, which were canceled in part by their inhibitors 5-(N-ethyl-N-isopropyl) amiloride (EIPA) and 3-methyladenine (3-MA). The macropinocytosis was induced by the Dkk-3 knock-down when there were sufficient extracellular nutrients. On the other hand, when the nutritional condition was poor, the autophagy was mainly induced by the Dkk-3 knock-down. These data indicated that Dkk-3 has a role in modulating macropinocytotic and autophagic pathways, a distinct function other than a Wnt antagonist.

Cite

CITATION STYLE

APA

Tsujimura, N., Yamada, N. O., Kuranaga, Y., Kumazaki, M., Shinohara, H., Taniguchi, K., & Akao, Y. (2016). A novel role of dickkopf-related protein 3 in macropinocytosis in human bladder cancer T24 cells. International Journal of Molecular Sciences, 17(11). https://doi.org/10.3390/ijms17111846

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free