Several methodologies for the preparation of polyethylene glycol-grafted immunoliposomes have been developed by attaching antibodies to the terminus of the polymer. Unilamellar liposomes were prepared containing a combination of a functionalized polyethylene glycol(3400) and an inert polyethylene glycol(2000) phosphatidylethanolamine derivate up to 5 mol%. The greater length of the functionalized polyethylene glycol derivate did not alter the liposomal sterical stability or the remote loading of doxorubicin. Anti-CD34 immunoliposomes were prepared by the reaction of maleimide-derivatized My10 antibody with generated thiol groups at the periphery of the liposomes and efficiencies of nearly 100% were obtained. The greater accessibility of the reactive group makes this strategy more efficient than others described. The immunoliposomes prepared bound specifically to CD34+ cells. Copyright (C) 1999 Elsevier Science B.V.
Mercadal, M., Domingo, J. C., Petriz, J., Garcia, J., & De Madariaga, M. A. (1999). A novel strategy affords high-yield coupling of antibody to extremities of liposomal surface-grafted PEG chains. Biochimica et Biophysica Acta - Biomembranes, 1418(1), 232–238. https://doi.org/10.1016/S0005-2736(99)00033-4