A novel strategy affords high-yield coupling of antibody to extremities of liposomal surface-grafted PEG chains

53Citations
Citations of this article
20Readers
Mendeley users who have this article in their library.

Abstract

Several methodologies for the preparation of polyethylene glycol-grafted immunoliposomes have been developed by attaching antibodies to the terminus of the polymer. Unilamellar liposomes were prepared containing a combination of a functionalized polyethylene glycol(3400) and an inert polyethylene glycol(2000) phosphatidylethanolamine derivate up to 5 mol%. The greater length of the functionalized polyethylene glycol derivate did not alter the liposomal sterical stability or the remote loading of doxorubicin. Anti-CD34 immunoliposomes were prepared by the reaction of maleimide-derivatized My10 antibody with generated thiol groups at the periphery of the liposomes and efficiencies of nearly 100% were obtained. The greater accessibility of the reactive group makes this strategy more efficient than others described. The immunoliposomes prepared bound specifically to CD34+ cells. Copyright (C) 1999 Elsevier Science B.V.

Cite

CITATION STYLE

APA

Mercadal, M., Domingo, J. C., Petriz, J., Garcia, J., & De Madariaga, M. A. (1999). A novel strategy affords high-yield coupling of antibody to extremities of liposomal surface-grafted PEG chains. Biochimica et Biophysica Acta - Biomembranes, 1418(1), 232–238. https://doi.org/10.1016/S0005-2736(99)00033-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free