Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos

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Abstract

Chicken sperm chromatin initiated an assembly of interphase-like nuclei in a cell-free cytoplasmic preparation from 1-6 h old Drosophila melanogaster embryos. The formation of these interphase-like nuclei from the condensed sperm chromatin happened in a series of distinct steps. Anti-Drosophila lamin monoclonal antibody stained the assembled nuclei in a pattern indistinguishable from normal Drosophila nuclei. This assembly process required an ATP regenerating system and could be blocked by the addition of novobiocin into the cell-free extract. © 1989.

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Ulitzur, N., & Gruenbaum, Y. (1989). Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos. FEBS Letters, 259(1), 113–116. https://doi.org/10.1016/0014-5793(89)81507-8

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